I have a project at this moment, and I had to disable the Bluetooth of my Raspberry Pi 3 to use the AMA0 port, I mean the main UART port. At this moment I need use both ports: AMA0 and ttyS0. Are there some form to put the ttyS0 on the GPIO again, using both UARTs at the same time?

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Unless you have a compute module you are limited to a single UART.

On some Pi's (not the Pi3) you can choose to use /dev/ttyAMA0 or /dev/ttyS0 but only ONE at a time as they use the same GPIO.

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